Dilution factor problem?
So I did these dilutions – 1:2, 1:4 and 1:10
I’m doing these in microliters. So for example, the 1:2 dilution was 500 microliters of this protein I’m using and 500 microliters of buffer. (Or also .5ml and .5ml, get it? Because each dilution is supposed to be 1ml total)
1:4 was 750 microliters and 250 microliters, then 1:10 was 900 microliters with 100 microliters.
So that went well and all. But now I need to do dilutions of 4:10, 325:100, and 175:100
And to this I say “What the hell?”
How many microliters then is that for the protein vs buffer for each?
This question is in the General Section. Responses must be helpful and on-topic.