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ScottyMcGeester's avatar

Dilution factor problem?

Asked by ScottyMcGeester (1897points) September 28th, 2012

So I did these dilutions – 1:2, 1:4 and 1:10

I’m doing these in microliters. So for example, the 1:2 dilution was 500 microliters of this protein I’m using and 500 microliters of buffer. (Or also .5ml and .5ml, get it? Because each dilution is supposed to be 1ml total)

1:4 was 750 microliters and 250 microliters, then 1:10 was 900 microliters with 100 microliters.

So that went well and all. But now I need to do dilutions of 4:10, 325:100, and 175:100

And to this I say “What the hell?”

How many microliters then is that for the protein vs buffer for each?

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