Public speakers - does the size of the crowd make you more nervous?

I have given various speeches, presentations, and talks over my career. Some were to groups of 20–30 people. Many have been to an audience of 250–300, and two were given to an audience of close to 1000 people.

The small and medium sized groups – not nervous at all. The larger group was more daunting to me, just because of its size. (And perhaps because there was a spotlight pointing on me as well, but that’s a different issue.) Even though I was talking about a professional issue in which I was very comfortable.

Does the size of an audience have a bearing on how at-ease you feel?