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Mr_Saturn512's avatar

Who is familiar with using thermal cyclers in a bio lab?

Asked by Mr_Saturn512 (558points) July 23rd, 2013

There’s this program that’s already set that I have to use on this thermal cycler labeled “CCC”. When I looked at the settings on the machine, the CCC program supposedly took a total of 22 hours. That’s not right. I mean, the procedure I’m doing is supposed to be done within a work day. I don’t understand how this is set like this. The annoying thing is nobody knows or remembers or really cares what the CCC program is supposed to be set to, yet I have to use it.

I’m using it on bacterial DNA from E.coli, digesting it with PstI restriction endonuclease. Basically, the entire procedure has me isolating DNA from E.coli cells through several steps of lysing and neutralizing and washing, and then I put it through PCR. How long do you think it should last in there?

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