General Question

bongo's avatar

What is the difference between the biological stain Alcian blue and Alcian blue 8GX?

Asked by bongo (4297 points ) May 17th, 2012

I have two bottles of Alcian blue one just says Alcian blue one says Alcian blue 8GX, which will be better for dissolving and use in the marking of fish for identification?

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4 Answers

bongo's avatar

And how do I make up a solution to stain fish for identification. What pH and strength would I require? There is so much variation in papers it is rather confusing!

thorninmud's avatar

There appears to be no difference. According to this, “Alcian blue 8GX” is the preferred name.

Lightlyseared's avatar

As far as I remember it’s the same stuff.

thorninmud's avatar

The most cited work on fish marking with Alcian blue seems to be Kelly, Marking freshwater and marine fish by injected dyes, 1967. He recommends a concentration of 64mg/ml.

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