Science Database about Bacteria?

Here is a PDF from the Journal of Biotechnology Research Center.

Thanks a ton for your help, I know you had to do a little searching for that. I have that paper and it’s what I am using for now.—It is a report on the finding for prodigiosin production within S. marcescens, not the growth of S. marcescens itself. It is a perfect example of what I mean by a paper simply stating the optimal temperature (see 2nd to last and 3rd to last line on forst page) but citing no source. —- this leads me to believe that this sort of thing is just common knowledge. All I need is something official that states it so I can cite it reliably.

This seems to touch upon what you are looking for.

“Optimally, Serratia marcescens grows at 37°C, but it can grow in temperatures that range from 5–40°C. ”

Thanks, yeah, I can’t use wikipedia.

It’s tough, I guess i’m looking for how that info was discovered in the first place, where does it come from.

@DrewJ Take the time to scour the sources…good luck

@Cruiser Coming up empty on that as well. I think I’ve settled in citing these sources I (we) found where the fact it just stated and referring to multiple and implying that it is “generally accepted” and hope my professor says thats okay. If not, I’ll ask other students how they handled this.

Appreciate the help, if nothing else, this helped me exhaust a resource and confirmed its not right unde rmy nose. Cheers.

There is a citation in the paper above for the line mentioned. Have you looked at the source? The Bergy

Bergy Manual of Determative Bacteriology

I found the Bergy Manual here

If you do a ctrl+f search for “marcescens” you’ll find after the second hit:
Optimum temperature, between 25° and
30° C. No growth at 37° C.

Total text of that section:

1. Serratia marcescens.

FAMILY IV. EXTEROBACTERIACEAE

361

2. Brilliant orange-red pellicle on plain gelatin.

2. Serratia indica.

B. Produce enough H2 with the CO2 from glucose to show gas in fermentation tubes.

1. Acetylmethjdcarbinol produced.

3. Serratia plymulhica.

2. Acetj’lmethylcarbinol not produced.

4. Serratia kiliensis.
II. Pigment soluble in water and in alcohol.

5. Serratia piscatorum.

1. Serratia niarcescens Bizio, 1823.
(Bizio, Polenta porporina, Biblioteca itali-
ana o sia Giornale de lettera, scienze e arti,
30, 1823, 288; Zoagalactina imetrofa Sette,
Memoria storico-naturale sull’arrossimento
straordinario di alcune sostanze alimentose
osservato nella provincia di Padova I’anno
1819. Venezia, 8°, 1824, 51; Monas prodigiosa
Ehrenberg, Bericht ii. d. z. Bekannt-ma-
chung geeigneten Verhandlungen d. Kgl.
preuss. Acad. d. Wissenschaften, 1849, 354.)

mar.ces’cens. L. part. adj. marcescens
pining away, decaying.

Description taken largely from Breed and
Breed (Jour. Bact., 9, 1924, 545).

Short rods, sometimes almost spherical,
0.5 by 0.5 to 1.0 micron, occurring singly
and occasionally in chains of 5 or 6 elements.
Motile by means of four peritrichous fla-
gella. Eight to ten flagella occur on cells
grown at 20° to 25° C. (de Rossi, Rivista
d’Igiene, I4, 1903, 000). Gram-negative.

Gelatin colonies: Thin, slightly granular,
gray becoming red, circular, slightly undu-
late. Medium liquefied rather quickly.

Gelatin stab: Infundibuliform liquefac-
tion. Sediment in liquefied medium usually
red on top, white in the depth.

Agar colonies: Circular, thin, granular,
white becoming red. R and S colonies with
mucoid variants (Reed, Jour. Bact., 34,
1937, 255).

Agar slant: White, smooth, moist layer,
taking on an orange-red to fuchsin color in
three or four days, sometimes with a metal-
lic luster.

Broth: Turbid; may form a red ring at
surface or slight pellicle; gray sediment.

Litmus milk: Acid reaction with soft co-
agulum. A red surface growth develops.
Little or no digestion takes place.

Potato : At first a white line appears which

rapidly turns red. The growth is lu.xuriant
and frequently shows a metallic luster.

Indole not produced.

Production of hj’drogen sulfide: Produced
from cysteine, cystine or organic sulfur com-
pounds containing either of these molecules.
Produced from sulfur but not from sulfites,
sulfates or thiosulfates (Tarr, Biochem.
Jour., 27, 1933, 1869; also see ibid., 28, 1934,
192).

Produces acetic, formic, succinic andlevo-
lactic acids, ethanol, acetylmethylcarbinol,
2,3-butylene glycol, CO2 and a trace of H2
from glucose (Pederson and Breed, Jour.
Bact., 16, 1928, 183).

Grows poorly or not at all in distilled
water containing urea, potassium chloride
and glucose.

Acetylmethylcarbinol is produced

(Breed) .

Nitrites produced from nitrates.

Pigment soluble in alcohol, ether, chlo-
roform, benzol and carbon bisulfide
(Schneider, Arb. bakt. Hochsch. Karlsruhe,
1, 1894, 210). Pigment may diffuse through
the agar, i.e., shows solubility in water
where strains are very deeply pigmented
(Breed). Pigment not produced at 35° C.

Sodium formate broth (Stark and Eng-
land, Jour. Bact., 29, 1935, 26) : Cultures do
not produce visible gas (Breed).

Odor of trimethylamine is produced.

Aerobic, facultatively anaerobic.

Optimum temperature, between 25° and
30° C. No growth at 37° C.

Source: Isolated by Bizio and Sette from
growth on corn meal mush (polenta).

Habitat: Found in water, soil, milk and
foods and in silk worms and other insects.

*Note, I’m not at all well-versed in this field, and I might be copy/pasting the wrong section.

^^ Figures a science officer would know where to find it. ^^

@Seek Brilliant man, just brilliant. That’s exactly what I need, I think. I’m guessing Bergy Manual is a credible source? Nevermind I’ll google that much. Thank you sir!!!

Cheers, lad.

@DrewJ What’s unclear to me is why you didn’t see the reference to the Bergy manual in the first place. It is properly referenced in the article that you said you had read and freely available on the internet. It is also the advice that @Cruiser gave you.

@Stinley The Bergy manual is something that came up but it’s not really free. I think what @Seek Found was someplace where all the text was ripped. That helped me confirm.

Not sure what you’re getting at though. maybe I just didn’t see it, that could be the case too

The Bergy manual is old enough that it is posted on Project Gutenberg. That is where I found it.

Just missed it. Thanks for the heads up on it though, this is why I asked in the first place. You’re a champ.